Click on Reference Sequences in the Table of Contents at the upper right of the gene record. If you know the gene symbol and species, enter them as follows: tpo sym AND human orgn Click on the desired gene. This plasmid is available through Addgene. Vector for expression of N-terminally FLAG-tagged proteins in mammalian cells. Include more flanking sequence > 50bp to obtain these scores. 2, 1, 28, 211932, 303120, RANBP3: maker-28-snap-gene-1.42, 170791, 224849. Warner Greene's lab contains the insert p52 and is published in Mol Cell Biol. The efficiency scores will instead be shown as '-'.
In other words, some guides may be fine, the problem may just be that the on-target is shown as an off-target.īecause there is no flanking sequence available, the guides in your sequence that are within 50bp of the ends will have no efficiency scores. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. In this case, the specificity scores of guide sequences are too low. This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Diseases associated with PER2 include Advanced Sleep Phase Syndrome, Familial, 1 and Advanced Sleep Phase Syndrome.
#Per2 snapgene software
When reading the list of guide sequences and off-targets below, bear in mind that in case that the input sequence is really in the genome and just has a few differences, the software will use the first found match as the on-target as it cannot distinguish 0-mismatch off-targets from 0-mismatch on-targets. PER2 (Period Circadian Regulator 2) is a Protein Coding gene.
Use a tool like BLAT to check if the sequence really has a 100% identical match in the target genome.
SnapGene can show predicted secondary structure for ssRNA sequences up to 3000 nucleotides in length. If not, you might want to check if you selected the right genome for your query sequence. Updated How do I view the predicted secondary structure of a single stranded RNA (ssRNA) sequence SnapGene 6.1 and later use the ViennaRNA package to calculate ssRNA secondary structure. Warning: The query sequence was not found in the selected genome. positive regulators and drive the expression of the negative regulators CRY1 and CRY2, as well as PER1, PER2, and PER3.
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